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solid phase sandwich enzyme linked immunosorbent assay kit  (R&D Systems)


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    R&D Systems solid phase sandwich enzyme linked immunosorbent assay kit
    Solid Phase Sandwich Enzyme Linked Immunosorbent Assay Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/solid+phase+sandwich+elisa+kit/Human+Proprotein+Convertase+9%2FPCSK9+DuoSet+ELISA/pm41469658-61-7-20
    Average 94 stars, based on 36 article reviews
    solid phase sandwich enzyme linked immunosorbent assay kit - by Bioz Stars, 2026-09
    94/100 stars

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    Sandwich ELISA:

    Article Title: Heart Failure Impacts Endothelial Cell Responses to Cardiac Surgery on Cardiopulmonary Bypass
    Article Snippet: .. ANG2 levels were measured using a commercially available Solid Phase Sandwich ELISA kit (R&D Systems, Minneapolis, MN, USA; SANG-20 + WA126) according to the manufacture’s instruction. ..

    Article Title: Reduced plasma interleukin-6 concentration after transcranial direct current stimulation to the prefrontal cortex.
    Article Snippet: Objectives: Transcranial direct stimulation (tDCS) targeted to the dorsolateral prefrontal cortex (DLPFC) reduces food intake and hunger, but its effects on circulating factors are unclear.. We assessed the effect of repeated administration of tDCS to the left DLPFC (L-DLPFC) on concentrations of pro/anti-inflammatory and appetitive hormone concentrations.. Materials and methods: Twenty-nine healthy adults with obesity (12 M; 42±11 y; BMI=39±8 kg/m2) received 3 consecutive inpatient sessions of either anodal or sham tDCS targeted to the L-DLPFC during a period of ad libitum food intake.

    Article Title: siRNA Delivery via Cross-Linked Gelatin Microparticles Enables Targeted Modulation of Osteogenic-Vascular Cross-Talk: An Advanced Human 3D in Vitro Test System for Therapeutic siRNA.
    Article Snippet: .. The amount of VEGF secreted was quantified by using a solid phase sandwich ELISA Kit (Human VEGF DuoSet ELISA, R&D Systems, Wiesbaden, Germany) according to the manufacturer’s protocol. ..

    Article Title: JAK/STAT3 represents a therapeutic target for colorectal cancer patients with stromal-rich tumors.
    Article Snippet: Material and methods Cell lines Cell lines utilized included DLD-1, HT29, SW620, HCT116, SW837 and CCD18Co which were obtained through American Type Tissue Culture (ATCC) and cultured according to guidelines. .. For detection of pSTAT3tyr705, a solid phase sandwich ELISA kit was utilized (DYC4607B-2, R&D Systems, Minneapolis, MN, USA). ..

    Article Title: JAK/STAT3 represents a therapeutic target for colorectal cancer patients with stromal-rich tumors
    Article Snippet: Cell lines utilized included DLD-1, HT29, SW620, HCT116, SW837 and CCD18Co which were obtained through American Type Tissue Culture (ATCC) and cultured according to guidelines. .. For detection of pSTAT3 tyr705 , a solid phase sandwich ELISA kit was utilized (DYC4607B-2, R&D Systems, Minneapolis, MN, USA). ..

    Article Title: Heart Failure Impacts Endothelial Cell Responses to Cardiac Surgery on Cardiopulmonary Bypass.
    Article Snippet: .. ANG2 levels were measured using a commercially available Solid Phase Sandwich ELISA kit (R&D Systems, Minneapolis, MN, USA; SANG-20 + WA126) according to the manufacture’s instruction. ..

    Article Title: siRNA Delivery via Cross‐Linked Gelatin Microparticles Enables Targeted Modulation of Osteogenic‐Vascular Cross‐Talk: An Advanced Human 3D in Vitro Test System for Therapeutic siRNA
    Article Snippet: .. The amount of VEGF secreted was quantified by using a solid phase sandwich ELISA Kit (Human VEGF DuoSet ELISA, R&D Systems, Wiesbaden, Germany) according to the manufacturer's protocol. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: siRNA Delivery via Cross-Linked Gelatin Microparticles Enables Targeted Modulation of Osteogenic-Vascular Cross-Talk: An Advanced Human 3D in Vitro Test System for Therapeutic siRNA.
    Article Snippet: .. The amount of VEGF secreted was quantified by using a solid phase sandwich ELISA Kit (Human VEGF DuoSet ELISA, R&D Systems, Wiesbaden, Germany) according to the manufacturer’s protocol. ..

    Article Title: siRNA Delivery via Cross‐Linked Gelatin Microparticles Enables Targeted Modulation of Osteogenic‐Vascular Cross‐Talk: An Advanced Human 3D in Vitro Test System for Therapeutic siRNA
    Article Snippet: .. The amount of VEGF secreted was quantified by using a solid phase sandwich ELISA Kit (Human VEGF DuoSet ELISA, R&D Systems, Wiesbaden, Germany) according to the manufacturer's protocol. ..



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    Analysis of siRNA silencing efficiency in osteogenic microtissues upon loading of cGM with siRNA‐carrying CaP‐NP and analysis of effects on osteogenic differentiation. a) Experimental setup: 1875 µL of siRNA‐loaded CaP‐NP were prepared and concentrated via ultrafiltration to a volume of 50 µL. Afterwards, 3.2 mg cGM (0.128 mg cGM/microtissue) were loaded with 100 µL of concentrated CaP‐NP. 10 000 hMSCs were then aggregated with 0.128 mg cGM, and osteogenic differentiation was induced by the addition of osteogenic supplements. Created in BioRender. Mitrach, F. (2025) b) siRNA‐mediated silencing efficiency of Chordin and WWP‐1 was quantified via gene expression levels over a period of 14 days of osteogenic differentiation and showed successful downregulation of both antagonists in osteogenic microtissues. c) Downstream effects of siRNA‐mediated antagonist silencing in microtissues were quantified via alkaline phosphatase activity on days 4 and 7, as well as d) calcium content as a measure of mineralization on day 18. e) Secretion of the important pro‐angiogenic <t>factor</t> <t>VEGF</t> by microtissues was quantified in cell culture supernatants using <t>ELISA</t> over 28 days of osteogenic differentiation. Data are presented as mean ± SD ( n = 4). Statistically significant differences are indicated with (∗) between the different groups ( p < 0.05), one‐way ANOVA with Tukey post hoc test. ALP : alkaline phosphatase; CaP‐NP : oligomer‐stabilized calcium phosphate nanoparticles; cGM : cross‐linked gelatin microparticles; DNA : deoxyribonucleic acid; hMSCs : human mesenchymal stem cells; mRNA : messenger RNA; n.d .: not detectable; pNP : para‐nitrophenyl; siRNA : small interfering RNA; VEGF : vascular endothelial growth factor.
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    Analysis of siRNA silencing efficiency in osteogenic microtissues upon loading of cGM with siRNA‐carrying CaP‐NP and analysis of effects on osteogenic differentiation. a) Experimental setup: 1875 µL of siRNA‐loaded CaP‐NP were prepared and concentrated via ultrafiltration to a volume of 50 µL. Afterwards, 3.2 mg cGM (0.128 mg cGM/microtissue) were loaded with 100 µL of concentrated CaP‐NP. 10 000 hMSCs were then aggregated with 0.128 mg cGM, and osteogenic differentiation was induced by the addition of osteogenic supplements. Created in BioRender. Mitrach, F. (2025) b) siRNA‐mediated silencing efficiency of Chordin and WWP‐1 was quantified via gene expression levels over a period of 14 days of osteogenic differentiation and showed successful downregulation of both antagonists in osteogenic microtissues. c) Downstream effects of siRNA‐mediated antagonist silencing in microtissues were quantified via alkaline phosphatase activity on days 4 and 7, as well as d) calcium content as a measure of mineralization on day 18. e) Secretion of the important pro‐angiogenic <t>factor</t> <t>VEGF</t> by microtissues was quantified in cell culture supernatants using <t>ELISA</t> over 28 days of osteogenic differentiation. Data are presented as mean ± SD ( n = 4). Statistically significant differences are indicated with (∗) between the different groups ( p < 0.05), one‐way ANOVA with Tukey post hoc test. ALP : alkaline phosphatase; CaP‐NP : oligomer‐stabilized calcium phosphate nanoparticles; cGM : cross‐linked gelatin microparticles; DNA : deoxyribonucleic acid; hMSCs : human mesenchymal stem cells; mRNA : messenger RNA; n.d .: not detectable; pNP : para‐nitrophenyl; siRNA : small interfering RNA; VEGF : vascular endothelial growth factor.
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    Analysis of siRNA silencing efficiency in osteogenic microtissues upon loading of cGM with siRNA‐carrying CaP‐NP and analysis of effects on osteogenic differentiation. a) Experimental setup: 1875 µL of siRNA‐loaded CaP‐NP were prepared and concentrated via ultrafiltration to a volume of 50 µL. Afterwards, 3.2 mg cGM (0.128 mg cGM/microtissue) were loaded with 100 µL of concentrated CaP‐NP. 10 000 hMSCs were then aggregated with 0.128 mg cGM, and osteogenic differentiation was induced by the addition of osteogenic supplements. Created in BioRender. Mitrach, F. (2025) b) siRNA‐mediated silencing efficiency of Chordin and WWP‐1 was quantified via gene expression levels over a period of 14 days of osteogenic differentiation and showed successful downregulation of both antagonists in osteogenic microtissues. c) Downstream effects of siRNA‐mediated antagonist silencing in microtissues were quantified via alkaline phosphatase activity on days 4 and 7, as well as d) calcium content as a measure of mineralization on day 18. e) Secretion of the important pro‐angiogenic <t>factor</t> <t>VEGF</t> by microtissues was quantified in cell culture supernatants using <t>ELISA</t> over 28 days of osteogenic differentiation. Data are presented as mean ± SD ( n = 4). Statistically significant differences are indicated with (∗) between the different groups ( p < 0.05), one‐way ANOVA with Tukey post hoc test. ALP : alkaline phosphatase; CaP‐NP : oligomer‐stabilized calcium phosphate nanoparticles; cGM : cross‐linked gelatin microparticles; DNA : deoxyribonucleic acid; hMSCs : human mesenchymal stem cells; mRNA : messenger RNA; n.d .: not detectable; pNP : para‐nitrophenyl; siRNA : small interfering RNA; VEGF : vascular endothelial growth factor.
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    Analysis of siRNA silencing efficiency in osteogenic microtissues upon loading of cGM with siRNA‐carrying CaP‐NP and analysis of effects on osteogenic differentiation. a) Experimental setup: 1875 µL of siRNA‐loaded CaP‐NP were prepared and concentrated via ultrafiltration to a volume of 50 µL. Afterwards, 3.2 mg cGM (0.128 mg cGM/microtissue) were loaded with 100 µL of concentrated CaP‐NP. 10 000 hMSCs were then aggregated with 0.128 mg cGM, and osteogenic differentiation was induced by the addition of osteogenic supplements. Created in BioRender. Mitrach, F. (2025) b) siRNA‐mediated silencing efficiency of Chordin and WWP‐1 was quantified via gene expression levels over a period of 14 days of osteogenic differentiation and showed successful downregulation of both antagonists in osteogenic microtissues. c) Downstream effects of siRNA‐mediated antagonist silencing in microtissues were quantified via alkaline phosphatase activity on days 4 and 7, as well as d) calcium content as a measure of mineralization on day 18. e) Secretion of the important pro‐angiogenic factor VEGF by microtissues was quantified in cell culture supernatants using ELISA over 28 days of osteogenic differentiation. Data are presented as mean ± SD ( n = 4). Statistically significant differences are indicated with (∗) between the different groups ( p < 0.05), one‐way ANOVA with Tukey post hoc test. ALP : alkaline phosphatase; CaP‐NP : oligomer‐stabilized calcium phosphate nanoparticles; cGM : cross‐linked gelatin microparticles; DNA : deoxyribonucleic acid; hMSCs : human mesenchymal stem cells; mRNA : messenger RNA; n.d .: not detectable; pNP : para‐nitrophenyl; siRNA : small interfering RNA; VEGF : vascular endothelial growth factor.

    Journal: Advanced Healthcare Materials

    Article Title: siRNA Delivery via Cross‐Linked Gelatin Microparticles Enables Targeted Modulation of Osteogenic‐Vascular Cross‐Talk: An Advanced Human 3D in Vitro Test System for Therapeutic siRNA

    doi: 10.1002/adhm.202504773

    Figure Lengend Snippet: Analysis of siRNA silencing efficiency in osteogenic microtissues upon loading of cGM with siRNA‐carrying CaP‐NP and analysis of effects on osteogenic differentiation. a) Experimental setup: 1875 µL of siRNA‐loaded CaP‐NP were prepared and concentrated via ultrafiltration to a volume of 50 µL. Afterwards, 3.2 mg cGM (0.128 mg cGM/microtissue) were loaded with 100 µL of concentrated CaP‐NP. 10 000 hMSCs were then aggregated with 0.128 mg cGM, and osteogenic differentiation was induced by the addition of osteogenic supplements. Created in BioRender. Mitrach, F. (2025) b) siRNA‐mediated silencing efficiency of Chordin and WWP‐1 was quantified via gene expression levels over a period of 14 days of osteogenic differentiation and showed successful downregulation of both antagonists in osteogenic microtissues. c) Downstream effects of siRNA‐mediated antagonist silencing in microtissues were quantified via alkaline phosphatase activity on days 4 and 7, as well as d) calcium content as a measure of mineralization on day 18. e) Secretion of the important pro‐angiogenic factor VEGF by microtissues was quantified in cell culture supernatants using ELISA over 28 days of osteogenic differentiation. Data are presented as mean ± SD ( n = 4). Statistically significant differences are indicated with (∗) between the different groups ( p < 0.05), one‐way ANOVA with Tukey post hoc test. ALP : alkaline phosphatase; CaP‐NP : oligomer‐stabilized calcium phosphate nanoparticles; cGM : cross‐linked gelatin microparticles; DNA : deoxyribonucleic acid; hMSCs : human mesenchymal stem cells; mRNA : messenger RNA; n.d .: not detectable; pNP : para‐nitrophenyl; siRNA : small interfering RNA; VEGF : vascular endothelial growth factor.

    Article Snippet: The amount of VEGF secreted was quantified by using a solid phase sandwich ELISA Kit (Human VEGF DuoSet ELISA, R&D Systems, Wiesbaden, Germany) according to the manufacturer's protocol.

    Techniques: Gene Expression, Activity Assay, Cell Culture, Enzyme-linked Immunosorbent Assay, Small Interfering RNA